Antagonists of Fomes annosus in the rhizosphere of grey alder (Alnus incana) and Norway spruce (Picea abies)     

M. Johansson  E. Marklund 《Forest Pathology》1980,10(7):385-395

A comparison was made of the fungistatic effects of the root microflora of grey alder and Norway spruce with Fomes annosus as test fungus. The significantly higher frequency of actinomycetes in the alder rhizosphere probably constituted the main difference in this respect. The importance of fungi seemed to be about equal in the rhizosphere of the two tree species. Methods for studies of antagonism are tested and discussed.  相似文献   

Stem volume equations and basic density for grey alder and common alder in Sweden   总被引：1，自引：0，他引：1  

Johansson  Tord 《Forestry》2005,78(3):249-262

The objective was to determine stem volume models for grey andcommon alders and, based on the models, stand volume for naturallyregenerated grey and common alder stands was summarized. Basicdensity for grey and common alders and mean annual growth forstands was estimated. Net volume accretion data were collectedfrom 24 stands of grey alder (Alnus incana (L.) Moench) and31 stands of common alder (Alnus glutinosa (L.) Gaertner) inSweden. The stands ranged in latitude from 58 to 64° N andfrom 56 to 62° N for grey and common alder, respectively.The mean age of grey and common alder stands was 41 years and48 years, respectively, the mean stand density 1726 stems ha^–1and 1078 stems ha^–1, and the mean diameter at breast height(over bark) was 20 cm and 21 cm. Stem volume equations weredeveloped for grey and common alders. The adopted model forgrey alder was based on diameter at breast height and height.For common alder, crown height was added to diameter and height.Mean standing volume (over bark) for grey and common alder standswas 428 and 374 m³ ha^–1. Mean annual growth for grey andcommon alder stands was 12.0 m³ and 8.4 m³ a^–1 ha^–1,respectively. Basic density (under bark), for grey and commonalder stems was 359 and 427 kg m^–3, respectively. Thebasic density (under bark) for the lowest twigs in the crownand in the lateral part of the crown was 415 and 421 kg m^–3for grey alder and 423 and 423 kg m^–3 for common alder.  相似文献   

Eating out of home and its correlates in 10 European countries. The European Prospective Investigation into Cancer and Nutrition (EPIC) study     

Orfanos P  Naska A  Trichopoulos D  Slimani N  Ferrari P  van Bakel M  Deharveng G  Overvad K  Tjønneland A  Halkjaer J  Santucci de Magistris M  Tumino R  Pala V  Sacerdote C  Masala G  Skeie G  Engeset D  Lund E  Jakszyn P  Barricarte A  Chirlaque MD  Martinez-Garcia C  Amiano P  Quirós JR  Bingham S  Welch A  Spencer EA  Key TJ  Rohrmann S  Linseisen J  Ray J  Boeing H  Peeters PH  Bueno-de-Mesquita HB  Ocke M  Johansson I  Johansson G  Berglund G  Manjer J  Boutron-Ruault MC  Touvier M  Clavel-Chapelon F  Trichopoulou A 《Public health nutrition》2007,10(12):1515-1525

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High-resolution protein structure determination by serial femtosecond crystallography     

Boutet S  Lomb L  Williams GJ  Barends TR  Aquila A  Doak RB  Weierstall U  DePonte DP  Steinbrener J  Shoeman RL  Messerschmidt M  Barty A  White TA  Kassemeyer S  Kirian RA  Seibert MM  Montanez PA  Kenney C  Herbst R  Hart P  Pines J  Haller G  Gruner SM  Philipp HT  Tate MW  Hromalik M  Koerner LJ  van Bakel N  Morse J  Ghonsalves W  Arnlund D  Bogan MJ  Caleman C  Fromme R  Hampton CY  Hunter MS  Johansson LC  Katona G  Kupitz C  Liang M  Martin AV  Nass K  Redecke L  Stellato F  Timneanu N  Wang D  Zatsepin NA  Schafer D  Defever J 《Science (New York, N.Y.)》2012,337(6092):362-364

Structure determination of proteins and other macromolecules has historically required the growth of high-quality crystals sufficiently large to diffract x-rays efficiently while withstanding radiation damage. We applied serial femtosecond crystallography (SFX) using an x-ray free-electron laser (XFEL) to obtain high-resolution structural information from microcrystals (less than 1 micrometer by 1 micrometer by 3 micrometers) of the well-characterized model protein lysozyme. The agreement with synchrotron data demonstrates the immediate relevance of SFX for analyzing the structure of the large group of difficult-to-crystallize molecules.  相似文献   

Efficiency of Two Enucleation Methods Connected to Handmade Cloning to Produce Transgenic Porcine Embryos     

J Li  K Villemoes  Y Zhang  Y Du  PM Kragh  S Purup  Q Xue  AM Pedersen  AL Jørgensen  JE Jakobsen  L Bolund  H Yang  G Vajta 《Reproduction in domestic animals》2009,44(1):122-127

The purpose of our work was to establish an efficient-oriented enucleation method to produce transgenic embryos with handmade cloning (HMC). After 41–42 h oocytes maturation, the oocytes were further cultured with or without 0.4 μg/ml demecolcine for 45 min [chemically assisted handmade enucleation (CAHE) group vs polar body (PB) oriented handmade enucleation (OHE) group respectively]. After removal of the cumulus cells and partial digestion of the zona pellucida, oocytes with visible extrusion cones and/or polar bodies attached to the surface were subjected to oriented bisection. Putative cytoplasts without extrusion cones or PB were selected as recipients. Two cytoplasts were electrofused with one transgenic fibroblasts expressing green fluorescent protein (GFP), while non-transgenic fibroblasts were used as controls. Reconstructed embryos were cultured in Well of Wells (WOWs) with porcine zygote medium 3 (PZM-3) after activation. Cleavage and blastocyst rates were registered on day 2 and day 7 of in vitro culture respectively. Meanwhile, the total blastocyst cell number was counted on day 7. We found that the difference was only observed between blastocyst rates (38.6 ± 2% vs 48.1 ± 3%) of cloned embryos with GFP transgenic fibroblast cells after CAHE vs OHE. With adjusted time-lapse for zonae-free cloned embryos cultured in WOWs with PZM-3, it was obvious that in vitro developmental competence after CAHE was compromised when compared with the OHE method. OHE enucleation method seems to be a potential superior alternative method used for somatic cell nuclear transfer (SCNT) with transgenic fibroblast cells.  相似文献   

Patterns of phenotypic diversity for phenologic and qualitative traits in Ethiopian tetraploid wheat germplasm     

Faris Hailu  Eva Johansson  Arnulf Merker 《Genetic Resources and Crop Evolution》2010,57(5):781-790

A total of 4,840 entries of tetraploid wheat germplasm collections representing 121 accessions from Ethiopia were evaluated for phenologic and qualitative trait diversity. The objectives were to assess the diversity patterns of the germplasm with respect to regions, species and altitudinal class. High values of Shannon–Weaver Diversity Index (H′) were recorded for most traits in each region, altitudinal classes, and species. Monomorphism was also high at accession levels. Both H′ and Nei’s gene diversity value for the entire data set (total gene diversity H _T = 0.572; the within accessions gene diversity H _S = 0.112; and gene diversity among accessions D _ST = 0.460) showed the variation for the trait is mainly among accessions/populations rather than within accessions/population. The least mean H′ value over all the traits used for the study was obtained from released varieties (among the origin groups) and Triticum dicoccon (among species). Triticum durum exhibit the highest H′ for a number of traits. Accessions collected from altitudinal class III (2401–2800 m a.s.l.) and class II (2001–2400 m a.s.l.) showed the highest H′ values for a valuable number of traits. Thus classifications using various phenology and qualitative traits enable to identify adaptation of a genotype and would improve the evaluation of genotype for potential adaptation.  相似文献   

Validation of human recombinant tissue factor-activated thromboelastography on citrated whole blood from clinically healthy dogs   总被引：1，自引：0，他引：1  

Wiinberg B  Jensen AL  Rojkjaer R  Johansson P  Kjelgaard-Hansen M  Kristensen AT 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2005,34(4):389-393

BACKGROUND: Thromboelastography (TEG) is an analytical method that enables global assessment of hemostatic function in whole blood (WB) with evaluation of both plasma and cellular components of hemostasis. TEG has a largely unused potential in the diagnostic workup and monitoring of dogs with hemostatic disorders and it may be a valuable supplement to traditional coagulation parameters. OBJECTIVES: The objective of this study was to establish a clinically applicable reference interval for a TEG assay using recombinant human tissue factor (TF) as the activator on citrated WB from clinically healthy dogs and to evaluate the stability of citrated WB stored for 30 minutes (T30) and 120 minutes (T120) at room temperature (RT). Additionally, we evaluated the analytical variation in reaction time (R), clotting time (K), angle (alpha), and maximum amplitude (MA). METHODS: Blood was collected from 18 clinically healthy dogs. Duplicate TEG analyses with TF as the activator at a concentration of 1:50,000 were performed on canine citrated WB at T30 and T120. R, K, a, and MAwere analyzed. RESULTS: Mean TEG values at T30/T120 were R = 5.61/4.91 minutes, K = 4.20/3.34 minutes, alpha = 45.33/50.90 degrees , and MA = 47.96/50.19 mm. Significant differences in these values were observed after storage for T30 and T120 at RT, with a tendency towards hypercoagulability at T120. The mean coefficients of variation were low. CONCLUSIONS: Canine citrated WB can be used for TEG analysis with human recombinant TF as the activator when stored at RT for T30 or T120. At both time points, the analytical variation was low, suggesting that TEG analysis may be of value in evaluating dogs with hemostatic disorders. A fixed time point should be chosen for serial measurements.  相似文献   

Detection of Renibacterium salmoninarum in tissue samples by sequence capture and fluorescent PCR based on the 16S rRNA gene     

Königsson MH  Ballagi A  Jansson E  Johansson KE 《Veterinary microbiology》2005,105(3-4):235-243

The 16S rRNA genes from eight isolates of Renibacterium salmoninarum with different origins and dates of isolation were sequenced to evaluate the possibility to construct a diagnostic PCR system with target sites within this gene. The sequences were found to be identical but for one single position in one of the isolates, and two regions with an adequate number of nucleotide differences as compared to closely related species were identified. Species-specific fluorescent PCR primers complementary to these regions were constructed as well as oligonucleotides for DNA preparation by sequence capture. A mimic molecule was constructed to be used as an internal control. The PCR was specific and allowed the detection of DNA equivalent to 1-10 R. salmoninarum genomes per reaction. The DNA preparation with sequence capture and analysis by PCR with a mimic was found to be a reliable method for analysis of kidneys from fish with BKD. The amount of PCR inhibiting substances present in the tissue was reduced, and the relevant DNA was concentrated in the capture step. Furthermore, the use of the mimic molecule in the system assured that false negative results could be identified.  相似文献   

Interleukin 6, serum amyloid A and haptoglobin as markers of treatment efficacy in pigs experimentally infected with Actinobacillus pleuropneumoniae     

Hultén C  Johansson E  Fossum C  Wallgren P 《Veterinary microbiology》2003,95(1-2):75-89

The possibility to use acute phase proteins to monitor the elimination of a bacterial infection in pigs would facilitate an objective assessment of treatment with various antimicrobial substances. To examine this possibility, the acute phase response (IL-6, serum amyloid A (SAA), and haptoglobin) elicited by Actinobacillus pleuropneumoniae and its reduction on treatment with various antibiotics was studied in serum from specific pathogen free (SPF) pigs. Pigs were infected intranasally with A. pleuropneumoniae serotype 2, and either left as non-treated control pigs or treated with different antibiotics intramuscularly at onset of respiratory disease (20h post-infection). Pigs responded to the infection with prominent increases in activity and concentrations of IL-6, SAA, and haptoglobin. These responses were to a certain extent overlapping and covered the time span from a few hours after infection until development of detectable levels of specific antibodies (7-10 days post-infection in untreated pigs). The haptoglobin response lasted until the end of the study on day 17 and thereby partly coincided with the antibody response. Treatment with antimicrobials that effectively reduced establishment of the infection with A. pleuropneumoniae also reduced the duration of all three acute phase responses, and reduced the concentration of serum haptoglobin. In contrast, less efficacious treatments did not reduce these acute phase responses. Thus, acute phase reactants can be applied to monitor therapeutic effects of antimicrobial drugs in the pig and measurements of IL-6, SAA and haptoglobin could add valuable information about the stage of infection during a disease outbreak.  相似文献   

Graduates in Veterinary Science     

Martin AM  Currie E. 《Australian veterinary journal》2004,82(8):496-496

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